Isolation of Protoplasts from Mesophyll cells of Morus alba L.
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چکیده
منابع مشابه
PLANT REGENERATION FROM MESOPHYLL PROTOPLASTS OF POTATO (SOLANUM TUBEROSUM L.) CULTIVAR DELAWARE USING SILVER THIOSULFATE (STS)
The use of in vitro potato shoot cultures for protoplast isolation is desirable for consistent protoplast qualities. Ethylene build-up in the culture vessels causes problems for leaf growth (e.g. decreasing leaf surface, dry weight and chlorophyll) in shoot culture. Fifty to one hundred µM Silver thiosulfate (STS) produced a larger leaf area as a source of plant material for protoplast isolatio...
متن کاملMesophyll cell protoplasts of potato: isolation, proliferation, and plant regeneration.
Mesophyll cell protoplasts were isolated from potato (Solanum tuberosum L. cv. Russet Burbank) leaves and induced to proliferate in culture. Protoplast division was observed only among preparations isolated from plants previously conditioned under short periods of low intensity illumination. Sustained growth and development of protoplast-derived calli (p-calli) occurred when they were maintaine...
متن کاملCharacterization of a New Flavone and Tyrosinase Inhibition Constituents from the Twigs of Morus alba L.
The twigs of Morus alba L. were found to show strong tyrosinase inhibition activity, and the responsible active components in the extract were further investigated in this study. A flavone, named morusone (1), and sixteen known compounds 2-17 were isolated from M. alba twigs and their structures were identified by interpretation of the corresponding ESI-MS and NMR spectral data. In the tyrosina...
متن کاملExtraction, purification and anti-fatigue activity of γ-aminobutyric acid from mulberry (Morus alba L.) leaves
Mulberry (Morus alba L.) is a tree species of Moraceae widely distributed in Southern China. In the present study, the white crystal of γ-aminobutyric acid (GABA) was purified from mulberry leaves, and its bioactivity was also investigated. The main results were as follows: first, the crude GABA was extracted from mulberry leaves by using biochemical methods. Then, the crude was purified by chr...
متن کاملplant regeneration from mesophyll protoplasts of potato (solanum tuberosum l.) cultivar delaware using silver thiosulfate (sts)
the use of in vitro potato shoot cultures for protoplast isolation is desirable for consistent protoplast qualities. ethylene build-up in the culture vessels causes problems for leaf growth (e.g. decreasing leaf surface, dry weight and chlorophyll) in shoot culture. fifty to one hundred µm silver thiosulfate (sts) produced a larger leaf area as a source of plant material for protoplast isolatio...
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ژورنال
عنوان ژورنال: Japanese Journal of Crop Science
سال: 1975
ISSN: 0011-1848,1349-0990
DOI: 10.1626/jcs.44.121